After his graduation, Henrik Dam became an instructor (assistant) in chemistry at the School of Agriculture and Veterinary Medicine. In 1923, he was appointed instructor in biochemistry at the Physiological Laboratory of the University of Copenhagen.
In 1925, he studied microchemistry at the University of Graz, Austria under the guidance of Fritz Pregl - the 1923 Nobel Prize Winner in Chemistry. In 1928, he became Assistant Professor at the Institute of Biochemistry, Copenhagen University.
Within a year in 1929, he was promoted to the position of Associate Professor at the same university.
In 1934, he received his Ph.D. degree in Biochemistry from the University of Copenhagen. His doctoral thesis was titled ‘Nogle Undersøgelser over Sterinernes Biologiske Betydning’ (Some Investigations on The Biological Significance of The Sterines).
On receiving Rockefeller Fellowship, he associated with Rudolph Schoenheimer in Freiburg, Germany in 1932–1933 and later with P. Karrer of Zurich, in 1935, to advance his study of sterines metabolism.
During this period, while studying the metabolism of sterines in chicken, he discovered vitamin K. He studied in details its occurrence, biological function in animals and plants, and application in human medicine.
He also investigated the fundamental chemical and physical properties of vitamin K. Later, he studied its purification and isolation in collaboration with P. Karrer.
From the study of vitamin K in animals some new symptoms were observed, such as increased capillary permeability and colouration of adipose tissue. This resulted from the intake of certain fats in the absence of vitamin E.
In 1940–1941, he went on a lecture tour to Canada and the United States under the sponsorship of the American Scandinavian Foundation. He researched at the Woods Hole Marine Biological Laboratories in 1941.
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From 1942–1945, he spent time as a Senior Research Associate at University of Rochester, New York, USA. It was during this period that he won the prestigious Nobel Prize in Physiology or Medicine in 1943. He became an Associate Member of the Rockefeller Institute for Medical Research in 1945.
While he was away to the U.S., he was appointed Professor of Biochemistry in 1941 at the Copenhagen Polytechnic Institute. He returned to Denmark in 1946, and continued his research on vitamin K, vitamin E, fats, cholesterol, and nutritional studies in relation to gall-stone formation.
In 1950, the designation of his Chair at the Polytechnic Institute of Copenhagen was changed to Professor of Biochemistry and Nutrition. From 1956 to 1962, he was the leader of the Biochemical Division of the Danish Fat Research Institute.
He published and also collaborated in the publication of more than 300 articles on biochemical problems; highlighting the biochemistry of sterols, vitamins K and E, and fats.
While studying sterines metabolism in chickens, Henrik Dam experimented by feeding the chicks a restricted, cholesterol-free diet. Within weeks, he noticed that the chickens bled easily and had reduced blood-clotting ability. He cured their illness by feeding them pigs' liver, alfalfa, cabbage, and spinach. He then discovered the dietary substance required for blood clotting and named it ‘coagulation vitamin’, or vitamin K.
Awards & Achievements
In 1939, both Henrik Dam and Edward Doisy independently isolated vitamin K in alfalfa. For this discovery, the duo shared the 1943 Nobel Prize in Physiology or Medicine.
He was a member of the Danish Academy of Technical Sciences in 1947 and the Royal Danish Academy of Sciences and Letters in 1948. He was Correspondant Étranger, Académie Royale de Médecine de Belgique in 1951.
He was elected Honorary Fellow of the Royal Society, Edinburgh in 1953 and became Joint Honorary President, International Union of Nutritional Sciences in 1954.
In 1960, he received the Norman Medal from the German Association for Fat Research and in 1961 he became a Corresponding Member of the German Association for Nutrition.